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2.2. Sertoli-NT2N-Aggregated-Cell (SNAC) Tissue Constructs Cells cultured in the rotating wall vessel (RWV) bioreactors, originally developed by NASA to simulate conditions of microgravity, organize into tissue constructs that express tissue-speci c markers [Becker et al., 1993; Lelkes et al., 1994, 1998]. This culture system has also been shown to effect the differentiation of cells grown in the RWV, as compared to those grown in conventional static cultures. For example, PC12 cells have been shown to aggregate and adopt a neuroendocrine phenotype when grown in the RWV, but not in conventional culture [Lelkes et al., 1998] (See Section 1.3). Similarly, neural stem cells [Wang and Good, 2001] and progenitor cells [Low et al., 2001] can be induced to differentiate when cultured in the RWV. We have previously reported aggregation of NT2 cells with Sertoli cells, using the High Aspect Ratio Vessel (HARV) RWV bioreactor to create a transplantable tissue construct that may provide a readily available source of dopaminergic neurons [Saporta et al., 2002].

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possible to pursue arable agriculture using only natural rainfall. In southern Europe, however (such as in the dry areas of Spain, Italy, and Greece), irrigation is necessary to sustain signi cant arable production. Consequently, in the latter areas groundwater use patterns conform more closely to the global norm, in which agricultural use of groundwater typically exceeds domestic by a factor of two to four. The scale of water demand varies considerably between the four use categories. Table 7.2 summarizes the typical demands associated with particular water uses within the four categories. Variation within each category is also considerable, depending on climate (for agricultural activities in particular), political priorities, and expectations. For instance, while most people in Europe would object if their personal access to water was

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In the same establishment, the following disbursements were made from the petty cash fund in November 2003: November 1 4 7 7 10 13 14 18 21 24 24 29 30 $ 3.07 14.20 1.30 12.00 0.32 11.50 4.60 11.00 3.26 4.02 1.16 10.50 1.16 office supplies flowers for lobby office supplies casual wages to cut the lawn postage due postage stamps COD parcel for owner taxi cost for owner collect telegram freight on linen delivery office supplies postage stamps (note there was no receipt for this) stamps

One of the standard problems in statistics is to decide between two alternative explanations for the data observed. For example, in medical testing, one may wish to test whether or not a new drug is effective. Similarly, a sequence of coin tosses may reveal whether or not the coin is biased. These problems are examples of the general hypothesis-testing problem. In the simplest case, we have to decide between two i.i.d. distributions. The general problem can be stated as follows: Problem 11.7.1 hypotheses:

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Pa1 : Access A1 relation at Site 1 Pa2 : Access A2 relation at Site 2 Pc1 : Access C1 relation at Site 1

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1. Lack of frequency synchronization among clocks at various network nodes; and 2. Phase wander and jitter on the digital bit streams.

The ShowComponents procedure shown in Listing 28-1 loops through each VBA component in the active workbook and writes the following information to a worksheet: The component s name The component s type The number of lines of code in the code module for the component

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